colevigo

Experiencia

e-mail: [email protected]
EDUCATION
2008: PhD in Biology. University of Santiago de Compostela, Santiago de Compostela, Spain. 'Comparison of immunological parameters among Ostrea edulis populations with different susceptibility to bonamiosis and the resistant species Crassostrea gigas'. Supervisors: Dr. Antonio Villalba Garcia, Dr. Asuncion Cao Hermida, Dr. Sandra Casas Liste.
RESEARCH EXPERIENCE
2003-2006: Pathology lab 'Centro de Investigacións Mariñas' (CIMA), Consellería de Pesca e Asuntos Marítimos, Xunta de Galicia, Spain
- Spectrophotometric techniques, ELISA and Western blot to measure immune parameters European flat oyster Ostrea edulis
- Comparison of the immune capability of Crassostrea gigas and O. edulis strains with different susceptibility to Bonamia ostreae
- Isolation and in vitro studies of the parasite B. ostreae
RESEARCH STAYS
2006: Institut Français de Recherche pour l'Exploration de la Mer (IFREMER), La Tremblade, France. Dr. Isabelle Arzul. PCR, cloning and sequencing to identify new DNA sequences of enzymes of the parasite B. ostreae. Measure of immunological parameters by flow cytometry, in contact-experiments between haemocytes of O. edulis and parasites. Three months
2005: Virginia Institute of Marine Science (VIMS), Gloucester Point, Virginia, USA. Dr. Ryan Carnegie. Molecular diagnostic techniques: in situ hybridisation technique for parasite detection. Collection of haemolymph samples from Crassostrea ariakensis for immunological assays. One month.
2004: University College Cork, Cork, Ireland. Dr Sarah Culloty. Molecular diagnostic techniques: DNA extraction and quantification and PCR assays for detection of B. ostreae. One month.
2001: Environmental monitoring grant. University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil and Spanish Agency of International Cooperation. Dr. Eduardo Lobo Alcayaga. Three months.
PUBLICATIONS
Comesaña P, Casas SM, Cao A, Abollo E, Villalba A. Respiratory burst in Ostrea edulis oysters with different susceptibility to bonamiosis and Crassostrea gigas. (in preparation).
Comesaña P, Casas SM, Cao A, Abollo E, Villalba A. Humoral and cellular enzymes in the haemolymph of Ostrea edulis oysters with different susceptibility to bonamiosis and Crassostrea gigas. (in preparation).
da Silva PM, Comesaña P, Fuentes J, Villalba A. Variability of haemocyte and haemolymph parameters in European flat oyster Ostrea edulis families obtained from brood stocks of different geographical origins and relation with infection by the protozoan Bonamia ostreae, Fish &Shellfish Immun doi:10.1016/j.fsi.2007.11.003
Abollo E, Ramilo A, Casas SM, Comesaña P, Cao, A, Carballal MJ, Villalba A. First detection of the protozoan parasite Bonamia exitiosa (Haplosporidia) infecting flat oyster Ostrea edulis grown in European waters, Aquaculture (2007), doi: 10.1016/j.aquaculture.2007.11.037